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Cardio Program of Excellence in Glycosciences

Upcoming Seminars

Cardio PEG Lab Meetings

(Physiology 612, 9.30-11)

This meeting is suspended over the summer. Watch this space for updates.


Cardio PEG Retreat

(Mt Washington Conference Center)

July 15th, Register by July 5th @ CardioPEG Registration

Schedule: Final Rev PEG 2016 Retreat Sched[1].pdfFinal Rev PEG 2016 Retreat Sched[1].pdf


Glycobiology Interest Group

This meeting is suspended over the summer. Watch this space for updates.

Upcoming Classwork

January 2017: Fundamentals of Glycobiology

July 2017: Techniques in Glycobiology

The O-GlcNAc core will provide the necessary reagents, specialized equipment, and expertise to study the O-GlcNAc modification in cardiac models, as well as providing a national resource for studying O-GlcNAcylation in physiological models of interest to the NHLBI. Specifically, the O-GlcNAc resources core will provide: 1) tools for detecting the O-GlcNAc modification and biosynthetic machinery; 2) glycopeptide synthesis; 3) development and characterization of O-GlcNAc specific antibodies; 4) assaying UDP-GlcNAc levels, and the activities of OGT and O-GlcNAcase; 5) tools for modulating O-GlcNAc levels in vivo and in vitro; and 6) advanced glycomics, for the detection, quantitation, and site-mapping of O-GlcNAcylation. Please note, that protocols used for the detection and analysis of the O-GlcNAc modification will regularly be uploaded onto this website and can be found under the protocols tab (left).

 
Resources available now: Reagents: Antibodies against O-GlcNAc, Antibodies against O-GlcNAcase and O-GlcNAc transferase; plasmids encoding OGT and O-GlcNAcase; Adenoviral expression of OGT and O-GlcNAcase; MEF cell line with for inducible OGT deletion; Techniques: Detecting O-GlcNAc (Click-it, Gal-transferase, Lectins, Antibodies); Measuring the activity of OGT and O-GlcNAcase; Peptide and Glycopetide synthesis; Enrichment of glycoproteins and glycopeptides (Click-it, Antibodies)

Requests should be made to:

gwhart@jhmi.edu or nzachara@jhmi.edu



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