Synopsis: This Core provides a critical link between glycoscientists and cardiologists and is key to the completion of all projects. The Cardiac and Myocyte Physiology Core is to provides: 1) isolation of adult and neonatal myocytes from rodent models, and physiologic analysis of myocyte systolic and diastolic function; 2) in vivo analysis of cardiovascular function based on non-invasive echo Doppler imaging; 3) comprehensive in vivo cardiac chamber functional analysis using pressure-volume relationships; 4) generation of infarction and preconditioning in isolated heart models and functional assessment in these models by Langendorff preparation; 5) steady-state force-calcium dependence in isolated skinned muscle trabeculae; 6) motoring reactive oxygen species (ROS) production by EPR, both in isolated cells and whole tissue levels; and 7) assessing the involvement of highly reactive cysteines in the biological action of several ROS and reactive nitrogen species (RNS).

Consultation and collaborations with the PEGs and community will be available.