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Cardio Program of Excellence in Glycosciences














Hui Zhang, Ph.D

PI

Address:

Contact information:

Johns Hopkins University

Department of Pathology

400 N. Broadway, Room 4011

Baltimore, MD 21287

Phone: 410 502 8149

Email: huizhang@jhu.edu

Website: http://pathology.jhu.edu/researchbrochure/facultysingle.cfm?id=3&facid=292

Hui Zhang CV 20160429.pdfHui Zhang CV 20160429.pdf














Nauder Faraday, M.D.

Co-PI
Associate Professor

Address:
Department of Anesthesiology/Critical Care Medicine
The Johns Hopkins University School of Medicine
600 North Wolfe Street, Meyer 298
Baltimore, MD 21287

Contact:
Phone: 410-955-9080
Fax: 410-955-8978
E-mail: nfaraday@jhmi.edu

 
PPC-faraday-cv-21jul11.docxPPC-faraday-cv-21jul11.docx

Project 4

Roles of Platelet Glycoproteins and Glycans on Platelet Reactivity and Cardiovascular Disease

Improving management of antiplatelet therapy is a significant issue for the millions of individuals with cardiovascular disease and for public health in general. The overall goal of this proposal is to characterize the glycoproteins and glycans in platelet cells in the response to aspirin treatment, and then dissect the critical glycoprotein structures that control and mediate the collagen adhesion and platelet reactivity. Understanding at the molecular level the platelet response to platelet activation could provide insights that can provide personalized treatment and development of new targets and therapies to control platelet reactivity. The significance of this information is its potential to inform the rational design of future anti-platelet therapy and to aid in the dissection of aspirin therapy failures and basic research in thrombosis.

Highlights​

 

Pingbo Zhang's work was nominated as one of the top 10 research advances for 2012 by the American Heart Association Functional Genomic and Translational Biology Council. Pingbo's paper "Reaction ion monitoring to identify site-specific troponin 1 phosphorylated residues in the failing human heart" is in press in Circulation. (Project 3) 

The CardioPEG published 26 papers in 2012. See News and Publications for a complete list.


Publications

Yang S, Chen L, Sun S, Shah P, Yang W, Zhang B, Zhang Z, Chan DW, Kass DA, van Eyk JE, Zhang H*. (2015) Glycoproteins identified from heart failure and treatment models. Proteomics 15, 567-579.

Yang S, Mishra S, Chen L, Zhou JY, Chan D, Chatterjee S and Zhang H*. (2015) Integrated Glycoprotein Immobilization Method for Glycopeptide and Glycan Analysis of Cardiac Hypertrophy. Analytical Chemistry 87, pp 9671–9678.

Shah P, Zhang B, Choi C, Yang S, Zhou J, Harlan R, Tian Y, Zhang Z, Chan DW, Zhang H*. (2015) Tissue Proteomics Using Chemical Immobilization and Mass Spectrometry. Analytical Biochemistry 469, 27-33.

Tian Y, Choi CH, Li QK, Rahmatpanah FB, Chen X, Kim SR, Veltri R, Chia D, Zhang Z, Mercola D, Zhang H*. (2015) Overexpression of periostin in stroma positively associated with aggressive prostate cancer. Plus One 10 (3), e0121502.

Tian Y, Almaraz RT, Choi CH, Li QK, Saeui C, Li D, Shah P, Bhattacharya R, Yarema KJ, and Zhang H*. (2015) Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells. Clinical Proteomics 12 (1), 1.

Toghi Eshghi S, Shah P, Yang W, Li X, Zhang H*. (2015) GPQuest: A Spectral Library Matching Algorithm for Site-Specific Assignment of Spectra from Tandem Mass Spectrometric Analysis of Intact Glycopeptides. Analytical Chemistry 87 (10), 5181-5188. 

Li Y, Shah P, DeMarzo A, VanEyk J, Li Q, Chan DW, Zhang H*. (2015) Identification of glycopeptides containing specific glycan motifs using a Lectin-Chemical Immobilization method. Analytical Chemistry 87 (9), 4683-4687.

Shah P, Wang X, Yang W, Toghi Eshghi S, Sun S, Hoti N, Pasay J, Rubin A, and Zhang H*. (2015) Integrated proteomic and glycoproteomic analyses of prostate cancer cells reveals glycoprotein alteration in protein abundance and glycosylation. Molecular & Cellular Proteomics 14 (10), 2753-2763.

Sun S, Zhang H*. (2015) Identification and validation of atypical N-glycosylation sites. Analytical chemistry 87 (24), 11948-11951.

Sun S, Zhang H*. (2015) Large-Scale Measurement of Absolute Protein Glycosylation Stoichiometry. Analytical chemistry 87 (13), pp 6479–6482.

Thomas SN*, Zhang H and Cotter RJ. (2015) Application of quantitative proteomics to the integrated analysis of the ubiquitylated and global proteomes of xenograft tumor tissues. Clinical Proteomics 1-15.

Thomas SN, Harlan R, Chen J, Aiyetan P, Liu Y, Sokoll LJ, Aebersold R, Chan DW, and Zhang H*. (2015) Multiplexed Targeted Mass Spectrometry-Based Assays for the Quantification of N-Linked Glycosite-Containing Peptides in Serum. Analytical chemistry 87 (21), pp 10830–10838.

Aiyetan P, Thomas SN, Zhang Z, Zhang H*. (2015) MRMPlus: an open source quality control and assessment tool for SRM/MRM assay development. BMC Bioinformatics 16: 411-418.

Kristine Y. DeLeon-Pennell, Yuan Tian, Bai Zhang, Courtney A. Cates, Rugmani Padmanabhan Iyer, Presley Cannon, Punit Shah, Paul Aiyetan, Ganesh V. Halade, Yonggang Ma, Elizabeth Flynn, Zhen Zhang, Yu-Fang Jin, Hui Zhang and Merry L. Lindse. (2015) CD36 Is a Matrix Metalloproteinase-9 Substrate that Stimulates Neutrophil Apoptosis and Removal during Cardiac Remodeling CIRCGENETICS.115.001249.

Yang S, Wang M, Chen L, Yin B, Song G, Turko I, Phinney K, Betenbaugh M, , Zhang H*, Shuwei Li*. (2015) QUANTITY: An Isobaric Tag for Quantitative Glycomics. Sci Rep. 5: 17585.

Yang S,Rubin A, Eshghi ST, Zhang H*. (2016) Chemoenzymatic method for glycomics: Isolation, identification, and quantitation. Proteomics 16(2), 241-256.

Sun S, Shah P, Toghi Eshghi S, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Uddin Höti N, Zhang Z, Chan DW and Zhang H*. (2016) Solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) for comprehensive characterization of glycoproteins. Nature Biotechnology 34, 84–88 (2016).


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