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Cardio Program of Excellence in Glycosciences

Protocols for the detection and analysis of glycoconjugates

 
The following documents are protocols commonly used by PEG groups for the detection and analysis of glycans. Note, video protocols are best viewed using the Safari web browser.
 
 
Nuclear&CytoplasmicFractionation
 
Protocol for fractionating frozen cells into nuclear and cytoplasmic fractions (Zachara Lab, Project 2)


O-GlcNAcase Assays: Prepared by Jungfeng Ma Ph.D. and Jennifer Groves (Project1 & Project 2)

Both a written protocol and video protocol are available.
131211_PEG_OGAassay_protocol_Groves.pdf131211_PEG_OGAassay_protocol_Groves.pdf

OGlcNAcase_Assay.mp4OGlcNAcase_Assay.mp4

Sugar Nucleotide Analysis: Prepared by Partha Bannerjee Ph.D. and Koen Raedschelders (Project 1 & GCF Fellow)

Both a written protocol and video protocol are available.


Sugar_Nucleotide_extraction_using_Methanol.docSugar_Nucleotide_extraction_using_Methanol.doc


Click Chemistry: Prepared by Sumita Mishra Ph.D. and Rahul Bhattacharya (Project 5 and Core C3)

Both a written protocol and video protocol are available.
Clickit_Alkyne.pdfClickit_Alkyne.pdf


A Protocol for purifying the O-GlcNAc specific antibody CTD110.6 (Zachara Lab, Project 2)
Antibodies_CTDPurification.docxAntibodies_CTDPurification.docx


A protocol for perfoming Methanol Chloroform precipitations (Zachara Lab, Project 2)
ProteinPrecipitation_MeOHChlorform.docxProteinPrecipitation_MeOHChlorform.docx


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