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Cardio Program of Excellence in Glycosciences

Techniques in Glycobiology (340.710):

Note: This course will run next in the summer of 2017.

Course Description:

Glycobiology, the discipline in which the structure, biosynthesis, and function of glycans is explored is rapidly emerging as the next growth area in biomedicine. Glycans, as saccharides, polysaccharides, glycolipids, glycosaminoglycans/proteoglycans, and glycoproteins, play fundamental roles in all organisms. In mammalian models, glycans have emerged as novel biomarkers of disease, regulators of signal transduction, are crucial for the development of novel vaccines and therapeutics, are essential for glycoprotein quality and control, are central to host-pathogen interactions, and play roles in the development of diabetes and cardiovascular disease. This course will provide students with a comprehensive training in the analysis of glycoconjugates, and where possible samples from disease models will be analyzed. 

This course involves a technical lecture in the morning, laboratory work during the day, and seminars in the evening.  Prerequisites: Students/fellows without a background in Glycobiology are encouraged to enroll in either "Introduction to Glycobiology" or “Fundamentals Glycobiology”. This is a 4 credit course, that takes 2 full weeks.

Please contact Natasha Zachara Ph.D. for more information (nzachara@jhmi.edu).


Course Schedule:


 

August 19th, 2013: Introduction to Glycoconjugate Analysis (Lecture)

Hart_OverviewLecture.pptxHart_OverviewLecture.pptx

Hart_OverviewLecture.pptxSmall.pdfHart_OverviewLecture.pptxSmall.pdf

August 21st, 2013: Colorimetric Assays (Lab)

August 23rd, 2013: Release of Glyccans from proteins and peptides (Lab)

August 26th, 2013: Analysis of the O-GlcNAc Modification (Lecture)

Lecture2_oglcnac_notes_sm.pdfLecture2_oglcnac_notes_sm.pdf

August 28th, 2013: Principles of Labeling Glycoconjugates/ Release of Glyccans from proteins and peptides (Lab)

August 30th. 2013: Proper use of Glyco-Enzyme Inhibitors (Lab)

Labor Day

September 4th, 2013: O-GlcNAc  (Lab)

September 6th, 2013: Sugar Nucleotide Analysis

September 9th, 2013: O-linked & N-linked Glycan Structure (Lecture)

September9th_NandOGlycans.pptxSeptember9th_NandOGlycans.pptx

September9th_NandOGlycans.pptx.pdfSeptember9th_NandOGlycans.pptx.pdf

September 11th, 2013: Glycosyltransferases as Probes (Lab)

September 13th, 2013: Separation of Oligosaccharides (Lab)

September 17th, 2013: Proteoglycans and GAGs (Lecture)

ProteoglycanLecture.pptxProteoglycanLecture.pptx
ProteoglycanLectureSmall.pdfProteoglycanLectureSmall.pdf

September 18th 2013: Bascis of Proteoglycan and GAG analysis 1 (Lab)

September 20th 2013: Bascis of Proteoglycan and GAG analysis 1 (Lab)

September 23rd, 2013: GPI Anchors (Lecture)

GPI_Analysis.pptxGPI_Analysis.pptx
GPI_Analysis.pptx.pdfGPI_Analysis.pptx.pdf

September 25th, 2013: GPI Anchors (Lab)

September 27th, 2013: Exoglycosidases to Probe Protein Structure (Lab)


September 30th, Monosaccharide and Linkage Analysis (Lecture)

Lecutre3_monosaccharides_slides.pptxLecutre3_monosaccharides_slides.pptx

October 2nd, Glycosylation Site Mapping (Workshop)

Lab_Siteassignment_slides_nz.pptxLab_Siteassignment_slides_nz.pptx

October 4th, Monosaccharide Analysis (Lab)

October 9th, 2013: Analysis of Glycoshingolipids (Lecture)

Analysis of Glycosphingolipids.pptxAnalysis of Glycosphingolipids.pptx

October 11th, 2013: Analysis of Glycoshingolipids (Lab)

October 11th, 2013: Lectin Arrays to Study Glycosylation (Workshop)

Lectin_Array_Lab_Introduction.pptxLectin_Array_Lab_Introduction.pptx

October 14th, 2013: Mass Spectrometry 1 (Lecture)

Mass Spectrometry Lab PEG Graham Van Eyk.pptxMass Spectrometry Lab PEG Graham Van Eyk.pptx

October 16th, 2013: MS Analysis of Glycoproteins and Glycans (Workshop)

MS and MS of glycoproteins Graham.pptxMS and MS of glycoproteins Graham.pptx

October 18th: MS Analysis of Glycans (Workshop and Lab)

N-glycan analysis.pptxN-glycan analysis.pptx

October 21st, 2013: Mass Spectrometry 2 (Lecture)

October21_MassSpec2.pptxOctober21_MassSpec2.pptx

October21_MassSpec2.pptx.pdfOctober21_MassSpec2.pptx.pdf

October 23rd, 2013: Principles of NMR (Lecture)

PegNmrcourse.pptPegNmrcourse.ppt

nmrlects.pdfnmrlects.pdf

October 25th, 2013: NMR Analysis of Glycoconjugates (Workshop)


Assessment: Studys will be asked to prepare "protocols" for publication in a manual (and will receive authorship credits) and to produce a video protocols.

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